The workflow of single-cell expression profiling using quantitative real-time PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00432501" target="_blank" >RIV/86652036:_____/14:00432501 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1586/14737159.2014.901154" target="_blank" >http://dx.doi.org/10.1586/14737159.2014.901154</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1586/14737159.2014.901154" target="_blank" >10.1586/14737159.2014.901154</a>
Alternative languages
Result language
angličtina
Original language name
The workflow of single-cell expression profiling using quantitative real-time PCR
Original language description
Biological material is heterogeneous and when exposed to stimuli the various cells present respond differently. Much of the complexity can be eliminated by disintegrating the sample, studying the cells one by one. Single-cell profiling reveals responsesthat go unnoticed when classical samples are studied. New cell types and cell subtypes may be found and relevant pathways and expression networks can be identified. The most powerful technique for single-cell expression profiling is currently quantitative reverse transcription real-time PCR (RT-qPCR). A robust RT-qPCR workflow for highly sensitive and specific measurements in high-throughput and a reasonable degree of multiplexing has been developed for targeting mRNAs, but also microRNAs, non-coding RNAs and most recently also proteins. We review the current state of the art of single-cell expression profiling and present also the improvements and developments expected in the next 5 years.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Expert Review of Molecular Diagnostics
ISSN
1473-7159
e-ISSN
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Volume of the periodical
14
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
323-331
UT code for WoS article
000335345600010
EID of the result in the Scopus database
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