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Evaluation of the expression of sperm proteins in normozoospermic and asthezoospermic sperm samples using monoclonal antibodies, flow cytometry and fluorescence microscopy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F15%3A00445182" target="_blank" >RIV/86652036:_____/15:00445182 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of the expression of sperm proteins in normozoospermic and asthezoospermic sperm samples using monoclonal antibodies, flow cytometry and fluorescence microscopy

  • Original language description

    Asthenozoospermia is one of the main seminal pathologies underlying male infertility. In our study we aimed to evaluate the ability of specific antibodies to detect the differential expression of selected protein markers by fluorescent microscopy and flow cytometry techniques. Therefore we analyzed sperm samples from 30 men with normal spermiograms and 30 men with asthenozoospermia (<40% motile spermatozoa) by the panel of our diagnostic anti-human sperm (Hs) antibodies. These antibodies were prepared in our laboratory and some of them are used in clinical practice as a tool for the differential diagnosis of various sperm pathologies. Both fluorescent microscopy and flow cytometry analysis revealed quantitative differences in the protein abundances between normozoospermic and asthenozoospermic sperm samples, namely, in GAPDHS, evaluated with Hs-8 MoAb, VCP (valosin-containing protein), evaluated with Hs-14 MoAb, and ATP synthase (cAMP-dependent protein kinase II, PRKAR2A), evaluated wi

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů