Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F16%3A00455666" target="_blank" >RIV/86652036:_____/16:00455666 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4103/1008-682X.151400" target="_blank" >http://dx.doi.org/10.4103/1008-682X.151400</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4103/1008-682X.151400" target="_blank" >10.4103/1008-682X.151400</a>

Result language

angličtina

Original language name

Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies

Original language description

Recent studies have shown that infertility affects estimated 15% of all couples. Male infertility is the primary or contributory cause in 60% of these cases. Consequently, the application of assisted reproduction is increasing. These methods could benefit from an extended evaluation of sperm quality. For this reason, we analyzed sperm proteins from 30 men with normal spermiograms and 30 men with asthenozoospermia. Ejaculates of both groups were tested by flow cytometry (FCM) and fluorescence with a setof well-characterized anti-human sperm Hs-monoclonal antibodies (MoAbs), which were generated in our laboratory. No statistically significant differences were found between normospermics and asthenospermics in the expression of the sperm surface proteinclusterin, evaluated with Hs-3 MoAb, and semenogelin, evaluated with Hs-9 MoAb. However, FCM revealed quantitative differences in the acrosomal proteins between normozoospermic and asthenozoospermic men, namely, in glyceraldehyde-3-phosph

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Asian Journal of Andrology

ISSN

1008-682X

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

1

Country of publishing house

IN - INDIA

Number of pages

6

Pages from-to

108-113

UT code for WoS article

000367846900018

EID of the result in the Scopus database

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