The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F16%3A00465660" target="_blank" >RIV/86652036:_____/16:00465660 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/16:00465660 RIV/00064190:_____/16:N0000162 RIV/00216208:11140/16:10326829 RIV/00216208:11110/16:10326829 and 2 more
Result on the web
<a href="http://dx.doi.org/10.1038/srep29023" target="_blank" >http://dx.doi.org/10.1038/srep29023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/srep29023" target="_blank" >10.1038/srep29023</a>

Result language
angličtina
Original language name
The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data
Original language description
Successful molecular analyses of human solid tissues require intact biological material with well-preserved nucleic acids, proteins, and other cell structures. Pre-analytical handling, comprising of the collection of material at the operating theatre, is among the first critical steps that influence sample quality. The aim of this study was to compare the experimental outcomes obtained from samples collected and stored by the conventional means of snap freezing and by PAXgene Tissue System (Qiagen). These approaches were evaluated by measuring rRNA and mRNA integrity of the samples (RNA Quality Indicator and Differential Amplification Method) and by gene expression profiling. The collection procedures of the biological material were implemented in two hospitals during colon cancer surgery in order to identify the impact of the collection method on the experimental outcome. Our study shows that the pre-analytical sample handling has a significant effect on the quality of RNA and on the variability of qPCR data. PAXgene collection mode proved to be more easily implemented in the operating room and moreover the quality of RNA obtained from human colon tissues by this method is superior to the one obtained by snap freezing.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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