Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10066" target="_blank" >RIV/00216208:11110/11:10066 - isvavai.cz</a>

Alternative codes found

RIV/00064165:_____/11:10066

Result on the web

<a href="http://pmr.cuni.cz/file/5587/pmr2011a0010.pdf" target="_blank" >http://pmr.cuni.cz/file/5587/pmr2011a0010.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue

Original language description

Early neonatal adaptation to extrauterine life is i.a. dependent on effective mitochondrial biogenesis during foetal development. Understanding of mitochondrial biogenesis is limited, because only scarce data are available from prenatal studies includingRNA analyses in human foetal tissues. Aims of the study were focused on the factors affecting RNA quality in human placental tissue (HPT) including temperature, time period before HPT freezing and the Apgar score. In addition, optimal reference genes were studied by analysing six candidate genes (ATP5O, SDHA, TBP, HPRT, PMBS, ATP6) for mRNA quantification by qPCR in HPT. Samples of HPT were obtained after the birth of 20 term neonates. Then RNA integrity, purity and yield were evaluated in dependence on the factors.The results showed that human placenta represents easily obtainable source of foetal tissue for studies concerning mitochondrial biogenesis. We demonstrated that the critical limit for optimal storage and handling of HPT are

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FG - Paediatrics

OECD FORD branch

—

Project

<a href="/en/project/NS10581" target="_blank" >NS10581: Genetic bases of cytochrome c oxidase deficiency</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Prague Medical Report

ISSN

1214-6994

e-ISSN

—

Volume of the periodical

112

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

93-101

UT code for WoS article

—

EID of the result in the Scopus database

—