Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F16%3A00472500" target="_blank" >RIV/86652036:_____/16:00472500 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/16:10329669

Result on the web

<a href="http://dx.doi.org/10.1242/bio.019265" target="_blank" >http://dx.doi.org/10.1242/bio.019265</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/bio.019265" target="_blank" >10.1242/bio.019265</a>

Result language

angličtina

Original language name

Identification and characterization of Xenopus tropicalis common progenitors of Sertoli and peritubular myoid cell lineages

Original language description

The origin of somatic cell lineages during testicular development is controversial in mammals. Employing basal amphibian tetrapod Xenopus tropicalis we established a cell culture derived from testes of juvenile male. Expression analysis showed transcription of some pluripotency genes and Sertoli cell, peritubular myoid cell and mesenchymal cell markers. Transcription of germline-specific genes was downregulated. Immunocytochemistry revealed that a majority of cells express vimentin and co-express Sox9 and smooth muscle alpha-actin (Sma), indicating the existence of a common progenitor of Sertoli and peritubular myoid cell lineages. Microinjection of transgenic, red fluorescent protein (RFP)-positive somatic testicular cells into the peritoneal cavity of X. tropicalis tadpoles resulted in cell deposits in heart, pronephros and intestine, and later in a strong proliferation and formation of cell-to-cell net growing through the tadpole body. Immunohistochemistry analysis of transplanted tadpoles showed a strong expression of vimentin in RFP-positive cells. No co-localization of Sox9 and Sma signals was observed during the first three weeks indicating their dedifferentiation to migratory-active mesenchymal cells recently described in human testicular biopsies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biology Open

ISSN

2046-6390

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

1275-1282

UT code for WoS article

000384077300011

EID of the result in the Scopus database

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