Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F17%3A00475858" target="_blank" >RIV/86652036:_____/17:00475858 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10363378

Result on the web

<a href="http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011" target="_blank" >http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/dx.doi.org/10.1016/j.theriogenology.2017.06.011" target="_blank" >dx.doi.org/10.1016/j.theriogenology.2017.06.011</a>

Result language

angličtina

Original language name

Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction.

Original language description

Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (a-L-fucosidase, a-D-galactosidase, b-D-galactosidase, b-D-N- ylhexosaminidase, and a-Dmannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of a-D-mannosidase, which was increased (P < 0.001), and b-D-galactosidase, which was decreased (P < 0.001) at neutral pH, and of a-D-galactosidase and b-N-acetylhexosaminidase, which were decreased (P < 0.0001) at the acidic pH. The comparison of glycosidases from follicular fluid and from blood plasma using red native electrophoresis revealed that most of the glycosidases are present in more than one isoenzyme form, some of them were detected mainly in the follicular fluid. Finally, we tested the effect of glycosidases on the interaction between zona pellucida and AWN 1 spermadhesin (putative sperm receptor of zona pellucida) and demonstrated that the effect of both b-D-galactosidase and to a lesser degree a-D-mannosidase led to a decrease in this interaction. We can hypothesize that these two glycosidases modulate the amount of zona pellucida oligosaccharide moieties and/or their structures for an optimal sperm binding in pigs.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theriogenelogy

ISSN

0093-691X

e-ISSN

—

Volume of the periodical

100

Issue of the periodical within the volume

Sep 15 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

80-87

UT code for WoS article

000406985600011

EID of the result in the Scopus database

2-s2.0-85020545089