Changes in microtubule overlap length regulate kinesin-14-driven microtubule sliding

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F17%3A00484511" target="_blank" >RIV/86652036:_____/17:00484511 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/NCHEMBIO.2495" target="_blank" >http://dx.doi.org/10.1038/NCHEMBIO.2495</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/NCHEMBIO.2495" target="_blank" >10.1038/NCHEMBIO.2495</a>

Result language

angličtina

Original language name

Changes in microtubule overlap length regulate kinesin-14-driven microtubule sliding

Original language description

Microtubule-crosslinking motor proteins, which slide antiparallel microtubules, are required for the remodeling of microtubule networks. Hitherto, all microtubule-crosslinking motors have been shown to slide microtubules at a constant velocity until no overlap remains between them, leading to the breakdown of the initial microtubule geometry. Here, we show in vitro that the sliding velocity of microtubules, driven by human kinesin-14 HSET, decreases when microtubules start to slide apart, resulting in the maintenance of finite-length microtubule overlaps. We quantitatively explain this feedback using the local interaction kinetics of HSET with overlapping microtubules that cause retention of HSET in shortening overlaps. Consequently, the increased HSET density in the overlaps leads to a density-dependent decrease in sliding velocity and the generation of an entropic force that antagonizes the force exerted by the motors. Our results demonstrate that a spatial arrangement of microtubules can regulate the collective action of molecular motors through the local alteration of their individual interaction kinetics.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Chemical Biology

ISSN

1552-4450

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1245-1252

UT code for WoS article

000415917900008

EID of the result in the Scopus database

2-s2.0-85034815412