Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00491034" target="_blank" >RIV/86652036:_____/18:00491034 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41467-018-04656-0" target="_blank" >http://dx.doi.org/10.1038/s41467-018-04656-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-018-04656-0" target="_blank" >10.1038/s41467-018-04656-0</a>

Result language
angličtina
Original language name
Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules
Original language description
Form and function of the mitotic spindle depend on motor proteins that crosslink microtubules and move them relative to each other. Among these are kinesin-14s, such as Ncd, which interact with one microtubule via their non-processive motor domains and with another via their diffusive tail domains, the latter allowing the protein to slip along the microtubule surface. Little is known about the influence of the tail domains on the protein's performance. Here, we show that diffusive anchorage of Ncd's tail domains impacts velocity and force considerably. Tail domain slippage reduced velocities from 270 nm s-1 to 60 nm s-1 and forces from several piconewtons to the sub-piconewton range. These findings challenge the notion that kinesin-14 may act as an antagonizer of other crosslinking motors, such as kinesin-5, during mitosis. It rather suggests a role of kinesin-14 as a flexible element, pliantly sliding and crosslinking microtubules to facilitate remodeling of the mitotic spindle.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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