Agrobacterium bohemicum sp nov isolated from poppy seed wastes in central Bohemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00491932" target="_blank" >RIV/86652036:_____/18:00491932 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00491875 RIV/00216208:11130/18:10375529 RIV/00216208:11310/18:10375529 RIV/00064203:_____/18:10375529
Result on the web
<a href="http://dx.doi.org/10.1016/j.syapm.2018.01.003" target="_blank" >http://dx.doi.org/10.1016/j.syapm.2018.01.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.syapm.2018.01.003" target="_blank" >10.1016/j.syapm.2018.01.003</a>
Alternative languages
Result language
angličtina
Original language name
Agrobacterium bohemicum sp nov isolated from poppy seed wastes in central Bohemia
Original language description
Two non-pathogenic strains R89-1 and R90(T) isolated from poppy seed (Papaver somniferum L.) wastes were phenotypically and genotypically characterized. Multilocus sequence analysis (MLSA) was conducted with six genes (atpD, ginA, gyrB, recA, rpoB, 16S rRNA). The strains represented a new species which clustered with Agrobacterium rubi NBRC 13261(T) and Agrobacterium skierniewicense Ch1 1(T) type strains. MLSA was further accompanied by whole-genome phylogeny, in silico DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses for both strains. ANI and dDDH values were deep below the species delineation threshold. Phenotypic features of the novel strains unequivocally allowed their differentiation from all other Agrobacterium species. Unlike other agrobacteria, the strains were salt sensitive and were able to biotransform morphine alkaloids. The dominant cellular fatty acids are 18:1 w7c, 16:0 and 12:0 aldehyde/16:1 iso I/14:0 30H summed in feature 2 and the major respiratory quinine is Q-10 (87%). The DNA G + C content is 56 mol%. Microbial community analysis indicated probable association with P. somniferum plant material. Altogether, these characteristics showed that strains R90(T) and R89-1 represent a new species of the genus Agrobacterium which we propose to name Agrobacterium bohemicum. The type strain of A. bohemicum is R90(T) (=CCM 8736(T) = DSM 104667(T)). (C) 2018 Elsevier GmbH. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/ED2.1.00%2F19.0390" target="_blank" >ED2.1.00/19.0390: Introduction of new research methods to BIOCEV</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Systematic and Applied Microbiology
ISSN
0723-2020
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
184-190
UT code for WoS article
000437220000004
EID of the result in the Scopus database
2-s2.0-85041295103