Interferons type II and their receptors R1 and R2 in fish species: Evolution, structure, and function

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00498869" target="_blank" >RIV/86652036:_____/18:00498869 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.fsi.2018.05.008" target="_blank" >http://dx.doi.org/10.1016/j.fsi.2018.05.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsi.2018.05.008" target="_blank" >10.1016/j.fsi.2018.05.008</a>

Result language

angličtina

Original language name

Interferons type II and their receptors R1 and R2 in fish species: Evolution, structure, and function

Original language description

Interferon gamma (IFN-gamma) is one of the key players in the immune system of vertebrates. The evolution and properties of IFN-gamma and its receptors in fish species are of special interest as they point to the origin of innate immunity in vertebrates. We studied the phylogeny, biophysical and structural properties of IFN-gamma and its receptors. Our phylogeny analysis suggests the existence of two groups of IFN-gamma related proteins, one specific for Acanthomorpha, the other for Cypriniformes, Characiformes and Silurifonnes. The analysis further shows an ancient duplication of the gene for IFN-gamma receptor 1 (IFN-gamma R1) and the parallel existence of the duplicated genes in all current teleost fish species. In contrast, only one gene can be found for receptor 2, IFN-gamma R2. The specificity of the interaction between IFN-gamma and both types of IFN-gamma R1 was determined by microscale thermophoresis measurements of the equilibrium dissociation constants for the proteins from three fish species. The measured preference of IFN-gamma for one of the two forms of receptor 1agrees with the bioinformatic analysis of the coevolution between IFN-gamma and receptor 1. To elucidate structural relationships between IFN-gamma of fish and other vertebrate species, we determined the crystal structure of IFN-gamma from olive flounder (Paralichthys olivaceus, PoliIFN-gamma) at crystallographic resolution of 2.3 angstrom and the low-resolution structures of Takifugu rubripes, Oreochromis niloticus, and Larimichthys crocea IFN-gamma by small angle X-ray diffraction. The overall PoliIFN-gamma fold is the same as the fold of the other known IFN-gamma structures but there are some significant structural differences, namely the additional C-terminal helix G and a different angle between helices C and D in PoliIFN-gamma.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish & Shellfish Immunology

ISSN

1050-4648

e-ISSN

—

Volume of the periodical

79

Issue of the periodical within the volume

AUG 2018

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

140-152

UT code for WoS article

000436918100017

EID of the result in the Scopus database

2-s2.0-85055519543