Alpha-Synuclein Aggregates Associated with Mitochondria in Tunnelling Nanotubes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F20%3A00533119" target="_blank" >RIV/86652036:_____/20:00533119 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s12640-020-00285-y" target="_blank" >https://link.springer.com/article/10.1007/s12640-020-00285-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12640-020-00285-y" target="_blank" >10.1007/s12640-020-00285-y</a>
Alternative languages
Result language
angličtina
Original language name
Alpha-Synuclein Aggregates Associated with Mitochondria in Tunnelling Nanotubes
Original language description
The interaction of alpha-synuclein with mitochondria in both typical and atypical Parkinson's disease is a critical component of degeneration. The mechanism of cell-to-cell propagation of pathological alpha-synuclein in synucleinopathies is unclear. Intercellular exchange of mitochondria along tunnelling nanotubes has been described in other diseases, such as cancer, however, its role in synucleinopathies is unknown. Pathological alpha-synuclein species have been demonstrated previously to move from cell to cell via tunnelling nanotubes. This process was further explored using co-culture and monoculture systems to determine if alpha-synuclein binds to migrating mitochondria within tunnelling nanotubes. Super-resolution analysis via stimulated emission depletion microscopy showed interaction between alpha-synuclein with the mitochondrial outer membrane and the presence of alpha-synuclein associated with mitochondria in tunnelling nanotubes between 1321N1, differentiated THP-1 and SH-SY5Y cell types. siRNA knockdown of Miro1, a critical protein-bridging mitochondria to the motor adaptor complex, had no effect on mitochondrial density or alpha-synuclein association with mitochondria in tunnelling nanotubes. The results show that alpha-synuclein aggregates associate with mitochondria in intercellular tunnelling nanotubes, suggesting that mitochondria-mediated alpha-synuclein transfer between cells may contribute to cell-to-cell spread of alpha-synuclein aggregates and disease propagation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Neurotoxicity Research
ISSN
1029-8428
e-ISSN
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Volume of the periodical
SEP 2020
Issue of the periodical within the volume
SEP 2020
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
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UT code for WoS article
000569293100002
EID of the result in the Scopus database
2-s2.0-85091009949