The Avian Retroviral Receptor Tva Mediates the Uptake of Transcobalamin Bound Vitamin B-12 (Cobalamin)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F21%3A00543825" target="_blank" >RIV/86652036:_____/21:00543825 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/21:00543825
Result on the web
<a href="https://journals-asm-org.d360prx.biomed.cas.cz/doi/epub/10.1128/JVI.02136-20" target="_blank" >https://journals-asm-org.d360prx.biomed.cas.cz/doi/epub/10.1128/JVI.02136-20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JVI.02136-20" target="_blank" >10.1128/JVI.02136-20</a>
Alternative languages
Result language
angličtina
Original language name
The Avian Retroviral Receptor Tva Mediates the Uptake of Transcobalamin Bound Vitamin B-12 (Cobalamin)
Original language description
The avian sarcoma and leukosis viruses (ASLVs) are important chicken pathogens. Some of the virus subgroups, including ASLV-A and K, utilize the Tva receptor for cell entrance. Though Tva was identified 3 decades ago, its physiological function remains unknown. Previously, we have noted an intriguing resemblance and orthology between the chicken gene coding for Tva and the human gene coding for CD320, a receptor involved in cellular uptake of transcobalamin (TC) in complex with vitamin B12/cobalamin (Cbl). Here, we show that both the transmembrane and the glycosylphosphatidylinositol (GPI)-anchored form of Tva in the chicken cell line DF-1 promotes the uptake of Cbl with the help of expressed and purified chicken TC. The uptake of TC-Cbl complex was monitored using an isotope-or fluorophore-labeled Cbl. We show that (i) TC-Cbl is internalized in chicken cells, and (ii) the uptake is lower in the Tva-knockout cells and higher in Tva-overexpressing cells when compared with that of wild-type chicken cells. The relation between physiological function of Tva and its role in infection was elaborated by showing that infection with ASLV subgroups (targeting Tva) impairs the uptake of TC-Cbl, while this is not the case for cells infected with ASLV-B (not recognized by Tva). In addition, exposure of the cells to a high concentration of TC-Cbl alleviates the infection with Tva-dependent ASLV.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Virology
ISSN
0022-538X
e-ISSN
1098-5514
Volume of the periodical
95
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e02136-20
UT code for WoS article
000636236300006
EID of the result in the Scopus database
—