Small RNA-Sequencing for Analysis of Circulating miRNAs Benchmark Study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F22%3A00558383" target="_blank" >RIV/86652036:_____/22:00558383 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/22:73615536 RIV/00216208:11310/22:10449511
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S1525157822000113?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1525157822000113?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmoldx.2021.12.006" target="_blank" >10.1016/j.jmoldx.2021.12.006</a>
Alternative languages
Result language
angličtina
Original language name
Small RNA-Sequencing for Analysis of Circulating miRNAs Benchmark Study
Original language description
Small RNA-sequencing (RNA-Seq) is being increasingly used for profiling of circulating microRNAs (miRNAs), a new group of promising biomarkers. Unfortunately, small RNA-Seq protocols are prone to biases limiting quantification accuracy, which motivated development of several novel methods. Here, we present comparison of all small RNA-Seq library preparation approaches that are commercially available for quantification of miRNAs in biofluids. Using synthetic and human plasma samples, we compared performance of traditional two-adaptor ligation protocols (Lexogen, Norgen), as well as methods using randomized adaptors (NEXTflex), polyadenylation (SMARTer), circularization (RealSeq), capture probes (EdgeSeq), or unique molecular identifiers (QIAseq). There was no single protocol outperforming others across all metrics. Limited overlap of measured miRNA profiles was documented between methods largely owing to protocol-specific biases. Methods designed to minimize bias largely differ in their performance, and contributing factors were identified. Usage of unique molecular identifiers has rather negligible effect and, if designed incorrectly, can even introduce spurious results. Together, these results identify strengths and weaknesses of all current methods and provide guidelines for applications of small RNA-Seq in biomarker research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Molecular Diagnostics
ISSN
1525-1578
e-ISSN
1943-7811
Volume of the periodical
24
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
386-394
UT code for WoS article
000804686600009
EID of the result in the Scopus database
2-s2.0-85127101175