MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00616698" target="_blank" >RIV/86652036:_____/24:00616698 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1534580723006512?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1534580723006512?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.devcel.2023.12.001" target="_blank" >10.1016/j.devcel.2023.12.001</a>
Alternative languages
Result language
angličtina
Original language name
MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement
Original language description
Microtubule doublets (MTDs) comprise an incomplete microtubule (Btubule) attached to the side of a complete cylindrical microtubule. These compound microtubules are conserved in cilia across the tree of life, however, the mechanisms by which MTDs form and are maintained in vivo remain poorly understood. Here, we identify microtubule-associated protein 9 (MAP9) as an MTD-associated protein. We demonstrate that C. elegans MAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. We find that loss of MAPH-9 causes ultrastructural MTD defects, including shortened and/or squashed Btubules with reduced numbers of protofilaments, dysregulated axonemal motor velocity, and perturbed cilia function. Because we find that the mammalian ortholog MAP9 localizes to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in regulating ciliary motors and supporting the structure of axonemal MTDs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
<a href="/en/project/GX19-27477X" target="_blank" >GX19-27477X: Cytoskeletal mechanics of the growth cone steering</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Developmental Cell
ISSN
1534-5807
e-ISSN
1878-1551
Volume of the periodical
59
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
24
Pages from-to
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UT code for WoS article
001177666300001
EID of the result in the Scopus database
2-s2.0-85182334372