Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F19%3A00521410" target="_blank" >RIV/86652036:_____/19:00521410 - isvavai.cz</a>
Result on the web
<a href="https://science.sciencemag.org/content/363/6424/285" target="_blank" >https://science.sciencemag.org/content/363/6424/285</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/science.aav2567" target="_blank" >10.1126/science.aav2567</a>
Alternative languages
Result language
angličtina
Original language name
Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails
Original language description
Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mechanism governing their formation is unknown. We used a cell-free assay to demonstrate a crucial inhibitory role of the carboxyl-terminal (C-terminal) tail of tubulin in MTD assembly. Removal of the C-terminal tail of an assembled A-microtubule allowed for the nucleation of a B-microtubule on its surface. C-terminal tails of only one A-microtubule protofilament inhibited this side-to-surface tubulin interaction, which would be overcome in vivo with binding protein partners. The dynamics of B-microtubule nucleation and its distinctive isotropic elongation was elucidated by using live imaging. Thus, inherent interaction properties of tubulin provide a structural basis driving flagellar MTD assembly.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science
ISSN
0036-8075
e-ISSN
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Volume of the periodical
363
Issue of the periodical within the volume
6424
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
285-288
UT code for WoS article
000456140700037
EID of the result in the Scopus database
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