Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F25%3A00617110" target="_blank" >RIV/86652036:_____/25:00617110 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/25:102442

Result on the web

<a href="https://www.mdpi.com/2076-2615/15/3/305" target="_blank" >https://www.mdpi.com/2076-2615/15/3/305</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani15030305" target="_blank" >10.3390/ani15030305</a>

Result language

angličtina

Original language name

Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges

Original language description

Spermatozoa are highly specialized male cells that are characterized by a unique ability to move, which is a critical factor for successful fertilization. The relative simplicity of motility assessment, especially in livestock, has made it a widely used parameter for evaluating ejaculate quality or cryopreserved semen in the clinical field, and an advanced tool in reproductive physiology and toxicology research. Technological advances in image analysis and computational methods have substantially increased its accuracy through the use of computer-assisted sperm analysis (CASA) to minimize subjective bias in motility assessments. Nevertheless, this more objective method still presents some significant challenges, including variability in the sample preparation, imaging conditions, and analytical parameters. These issues contribute to inconsistency and impair the reproducibility and comparability of data between laboratories. The implementation of standardized protocols, combined with comprehensive training and rigorous evaluation, can serve to mitigate some of the emerging inconsistencies. In addition, the in vitro conditions under which CASA analyses are performed often differ significantly from the natural environment of the female reproductive tract in vivo. This review discusses the methodologies, critical issues, and limitations of sperm motility analyses using CASA, with a particular focus on the boar as an important agricultural and biomedical model species in which this system is widely used.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

<a href="/en/project/GA22-31156S" target="_blank" >GA22-31156S: Key molecules involved in gamete maturation and sperm-zona pellucida recognition in pig and cattle</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Volume of the periodical

15

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

34

Pages from-to

305

UT code for WoS article

001418449200001

EID of the result in the Scopus database

2-s2.0-85217792325