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Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F16%3A00460374" target="_blank" >RIV/86652079:_____/16:00460374 - isvavai.cz</a>

  • Alternative codes found

    RIV/70883521:28140/16:43874682

  • Result on the web

    <a href="http://dx.doi.org/10.1007/978-3-319-32034-2_34" target="_blank" >http://dx.doi.org/10.1007/978-3-319-32034-2_34</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-3-319-32034-2_34" target="_blank" >10.1007/978-3-319-32034-2_34</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures

  • Original language description

    This work deals with an automatic quantitative analysis of photosynthetic cell cultures. It uses images captured by a confocal fluorescent microscope for automatic determination the number of cells in sample containing complex 3D structure of cell clusters. Experiments were performed on the confocal microscope Leica TCS SP8 X. The cell nuclei were stained by SYBR Green fluorescent DNA binding marker. In the first step we used combination of adaptive thresholding to found out areas where nuclei were located. Proposed segmentation steps allowed reduction of noise and artefacts. Z-axis position was obtained as a location of peak from intensity profile. Finally model of scene can be created by emplacement of spheres with adequate diameter to found 3D coordinates. Number of cells per volumetric unit were determined in structurally different culture samples of cell cultures Chenopodium rubrum (Cr) and Solanum lycopersicum (To). The results were verified by manual counting

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EH - Ecology - communities

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 – Development of the Centre of Global Climate Change Impacts Studies</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Hybrid Artificial Intelligent Systems: 11th International Conference, HAIS 2016, Seville, Spain, April 18-20, 2016, Proceedings

  • ISBN

    978-3-319-32033-5

  • ISSN

    0302-9743

  • e-ISSN

  • Number of pages

    12

  • Pages from-to

    402-103

  • Publisher name

    Springer International Publishing

  • Place of publication

    Cham

  • Event location

    Seville

  • Event date

    Apr 18, 2016

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article