Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F23%3A00579023" target="_blank" >RIV/86652079:_____/23:00579023 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/23:00133396 RIV/65269705:_____/23:00078512 RIV/00159816:_____/23:00078512

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fmolb.2023.1254699/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmolb.2023.1254699/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmolb.2023.1254699" target="_blank" >10.3389/fmolb.2023.1254699</a>

Result language

angličtina

Original language name

Detection of cell-free histones in the cerebrospinal fluid of pediatric central nervous system malignancies by imaging flow cytometry

Original language description

Introduction: Pediatric brain tumours (PBT) are one of the most common malignancies during childhood, with variable severity according to the location and histological type. Certain types of gliomas, such a glioblastoma and diffuse intrinsic pontine glioma (DIPG), have a much higher mortality than ependymoma and medulloblastoma. Early detection of PBT is essential for diagnosis and therapeutic interventions. Liquid biopsies have been demonstrated using cerebrospinal fluid (CSF), mostly restricted to cell free DNA, which display limitations of quantity and integrity. In this pilot study, we sought to demonstrate the detectability and robustness of cell free histones in the CSF.Methods: We collected CSF samples from a pilot cohort of 8 children with brain tumours including DIPG, medulloblastoma, glioblastoma, ependymoma and others. As controls, we collected CSF samples from nine children with unrelated blood malignancies and without brain tumours. We applied a multichannel flow imaging approach on ImageStream(X) to image indiviual histone or histone complexes on different channels.Results: Single histones (H2A, macroH2A1.1, macroH2A1.2 H2B, H3, H4 and histone H3 bearing the H3K27M mutation), and histone complexes are specifically detectable in the CSF of PBT patients. H2A and its variants macroH2A1.1/macroH2A1/2 displayed the strongest signal and abundance, together with disease associated H3K27M. In contrast, mostly H4 is detectable in the CSF of pediatric patients with blood malignancies.Discussion: In conclusion, free histones and histone complexes are detectable with a strong signal in the CSF of children affected by brain tumours, using ImageStream(X) technology and may provide additive diagnostic and predictive information.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/NU23-03-00318" target="_blank" >NU23-03-00318: Free circulating histone complexes as diagnostic tool for central nervous system (CNS) pediatric cancers.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in molecular biosciences

ISSN

2296-889X

e-ISSN

2296-889X

Volume of the periodical

10

Issue of the periodical within the volume

NOV

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

1254699

UT code for WoS article

001101920700001

EID of the result in the Scopus database

2-s2.0-85176564687