A quantitative description of light-limited cyanobacterial growth using flux balance analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F24%3A00599393" target="_blank" >RIV/86652079:_____/24:00599393 - isvavai.cz</a>
Result on the web
<a href="https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1012280" target="_blank" >https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1012280</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pcbi.1012280" target="_blank" >10.1371/journal.pcbi.1012280</a>
Alternative languages
Result language
angličtina
Original language name
A quantitative description of light-limited cyanobacterial growth using flux balance analysis
Original language description
The metabolism of phototrophic cyanobacteria is an integral part of global biogeochemical cycles, and the capability of cyanobacteria to assimilate atmospheric CO2 into organic carbon has manifold potential applications for a sustainable biotechnology. To elucidate the properties of cyanobacterial metabolism and growth, computational reconstructions of genome-scale metabolic networks play an increasingly important role. Here, we present an updated reconstruction of the metabolic network of the cyanobacterium Synechocystis sp. PCC 6803 and its quantitative evaluation using flux balance analysis (FBA). To overcome limitations of conventional FBA, and to allow for the integration of experimental analyses, we develop a novel approach to describe light absorption and light utilization within the framework of FBA. Our approach incorporates photoinhibition and a variable quantum yield into the constraint-based description of light-limited phototrophic growth. We show that the resulting model is capable of predicting quantitative properties of cyanobacterial growth, including photosynthetic oxygen evolution and the ATP/NADPH ratio required for growth and cellular maintenance. Our approach retains the computational and conceptual simplicity of FBA and is readily applicable to other phototrophic microorganisms.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Computational Biology
ISSN
1553-734X
e-ISSN
1553-7358
Volume of the periodical
20
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
27
Pages from-to
e1012280
UT code for WoS article
001422919200008
EID of the result in the Scopus database
2-s2.0-85200576529