Sustained CREB phosphorylation by lipid-peptide liquid crystalline nanoassemblies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2FCZ______%3A_____%2F23%3AN0000050" target="_blank" >RIV/CZ______:_____/23:N0000050 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s42004-023-01043-9#Ack1" target="_blank" >https://www.nature.com/articles/s42004-023-01043-9#Ack1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42004-023-01043-9" target="_blank" >10.1038/s42004-023-01043-9</a>
Alternative languages
Result language
angličtina
Original language name
Sustained CREB phosphorylation by lipid-peptide liquid crystalline nanoassemblies
Original language description
"Cyclic-AMP-response element-binding protein (CREB) is a leucine zipper class transcription factor that is activated through phosphorylation. Ample CREB phosphorylation is required for neurotrophin expression, which is of key importance for preventing and regenerating neurological disorders, including the sequelae of long COVID syndrome. Here we created lipid-peptide nanoassemblies with different liquid crystalline structural organizations (cubosomes, hexosomes, and vesicles) as innovative nanomedicine delivery systems of bioactive PUFA-plasmalogens (vinyl ether phospholipids with polyunsaturated fatty acid chains) and a neurotrophic pituitary adenylate cyclase-activating polypeptide (PACAP). Considering that plasmalogen deficiency is a potentially causative factor for neurodegeneration, we examined the impact of nanoassemblies type and incubation time in an in vitro Parkinson's disease (PD) model as critical parameters for the induction of CREB phosphorylation. The determined kinetic changes in CREB, AKT, and ERK-protein phosphorylation reveal that non-lamellar PUFA-plasmalogen-loaded liquid crystalline lipid nanoparticles significantly prolong CREB activation in the neurodegeneration model, an effect unattainable with free drugs, and this effect can be further enhanced by the cell-penetrating peptide PACAP. Understanding the sustained CREB activation response to neurotrophic nanoassemblies might lead to more efficient use of nanomedicines in neuroregeneration. PUFA-plasmalogens show neuroprotective properties via the stimulation of CREB activation, however, their efficiency is limited by low bioavailabilities. Here, the authors develop PUFA-plasmalogen-loaded liquid crystalline lipid-peptide nanoparticles to achieve sustained CREB activation in an in vitro neurodegeneration model."
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Communications Chemistry
ISSN
2399-3669
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
241 (1-16)
UT code for WoS article
001099053800002
EID of the result in the Scopus database
2-s2.0-85175858452