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Human rotavirus A detection: Comparison of enzymatic immunoassay and rapid chromatographic test with two quantitative RT-PCR assays
with results of commercial quantitative reverse-transcription PCR (RT-qPCR) test test (RDT) (IMMUNOQUICK® No-Rot-Adeno). As a reference method a commercial RT-qPCR-qPCR test pre...
Virology
- 2018 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Using nanofiber filter technology to detect pathogens in fish farms
of the technology was carried out using RT-qPCR diagnostics on 5 kits developed by the team: RT-qPCR AEROMident, RT-qPCR FLAVident, RT-qPCR ICHTident, RT-qPCR ...
Fishery
- 2024 •
- Ztech •
- Link
Rok uplatnění
Ztech - Ověřená technologie
Výsledek na webu
Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR
% tested positive for SARS-CoV-2 using nasopharyngeal swab sampling. Our direct RT-qPCR of painless nasal-swab self-collection and direct RT-qPCR for mass testing during the SARS approach using pa...
Zoology
- 2021 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Direct-RT-qPCR detection of SARS-CoV-2 without RNA extraction as part of a COVID-19 testing strategy: from sample to result in one hour
suggested such a testing programme that includes a rapid RT-qPCR approach without RNA extraction. The Direct-One-Step-RT-qPCR (DIOS-RT-qPCR) assay detects SARS-CoV-2 in less. The limit o...
Immunology
- 2020 •
- JSC •
- Link
Rok uplatnění
JSC - Článek v periodiku v databázi SCOPUS
Výsledek na webu
An integrated microfluidic platform for nucleic acid testing
polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely alig...
Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])
- 2024 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
An integrated microfluidic platform for nucleic acid testing.
polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely alig...
Electrical engineering, Electronic engineering, Information engineering
- 2024 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Impact of normalization method on experimental outcome using RT qPCR in Staphylococcus aureus
The expression study of the enterotoxin G gene served as an example how unsuitable choice of reference genes could change results of an RT-qPCR experiment. The review summarizing how far this problem is widespread in the literature ...
EE - Mikrobiologie, virologie
- 2012 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
-step RT-qPCR with SYBR-green detection chemistry called Two-tailed RT-qPCR of the position of the mismatched nucleotide. Two-tailed RT-qPCR has a dynamic range of seven and the miRNA profiles mea...
Genetics and heredity (medical genetics to be 3)
- 2017 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Evaluation of miRNA detection methods for the analytical characteristics necessary for clinical utilization
observed a different absolute concentration range in RT-qPCR (fmol/mu l) and methods robustness (CV 8-19%) than RT-qPCR (CV 39-50%). The calibration curve in TaqMan Advanced technologies were compared: 1) RT
Biochemistry and molecular biology
- 2019 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
TATAA courses – 2 Days qPCR for miRNA analysisnn
analysis in biofluidsn• Hands-on lab testing of quality and quantity of miRNAnDay 2n• MicroRNA quantification with microarray, nanoString, NGS and RT -qPCR methodsn• Comparison of miRNA quantification methodsn• Experimenta...
Biochemistry and molecular biology
- 2018 •
- W
Rok uplatnění
W - Uspořádání workshopu
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