TATAA courses – 2 Days qPCR for miRNA analysisnn

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00496888" target="_blank" >RIV/86652036:_____/18:00496888 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
TATAA courses – 2 Days qPCR for miRNA analysisnn
Original language description
This course introduces miRNA and its variant forms. Methods to extract and purify microRNA and prepare it for analysis, including quality control. Methods for microRNA expression analysis are presented and compared, to learn how to too choose the best method for each study. Analysis of microRNA data is also discussed, including challenging normalization. The course contains:nDay 1n• MicroRNA background including biogenesis and functionn• MicroRNA extraction – sampling, miRNA stability, extraction kit comparisonn• Quality Control of miRNAn• MicroRNA and liquid biopsies – challenges of miRNA analysis in biofluidsn• Hands-on lab testing of quality and quantity of miRNAnDay 2n• MicroRNA quantification with microarray, nanoString, NGS and RT -qPCR methodsn• Comparison of miRNA quantification methodsn• Experimental design and data analysisn• RT-qPCR normalization of miRNA datan• Introduction to NGS data analysisn• Hands-on lab of miRNA RT-qPCR analysis including data analysis in Genexn
Czech name
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Czech description
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Project
<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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