Structural and functional analysis of interaction between calmodulin and plectin isoform 1a

Provider

Ministry of Education, Youth and Sports

Programme

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Call for proposals

FP6-2004-Mobility-12

Main participants

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Contest type

RP - Co-financing of EC programme

Contract ID

MSMT-5278/2013-311

Project name in Czech

Strukturně- funkční analýza interakce mezi kalmodulinem a plektinem 1a

Annotation in Czech

The major objective of this project is to decipher the structural basis of the interaction between the calmodulin and the N-terminal actin binding domain of plectin isoform 1a and to provide the model with a functional-structural basis. Following goals will be pursued: i) To investigate interaction of calmodulin with the N-terminal domain of plectin isoform 1a (1aABD) - structural analysis of the interaction between calmodulin and N-terminal domain of plectin isoform 1a ii) To study the mechanism of calmodulin-dependent disruption of the interaction between plectin and integrin - analysis of the disassembly mechanism of intergrin-plectin complex after binding of calmodulin iii) To address the role of calmodulin in modulation of the function of actin binding domains - analysis and comparison of ABDs from a selected number of actin binding proteins, which calmodulin and CaM-like domain bind. iv) To test and validate proposed model of calmodulin-plectin 1a interaction ex vivo - structural analysis of theinteraction between calmodulin and N-terminal domain of plectin isoform 1a and analysis of the disassembly mechanism of intergrin-plectin complex after binding of calmodulin ex vivo.

R&D category

ZV - Basic research

CEP classification - main branch

EB - Genetics and molecular biology

CEP - secondary branch

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CEP - another secondary branch

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OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics

Provider evaluation

U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

Project results evaluation

This project is being realized in the framework of the MOBILITY Activity that aims primarily on establishing and strenghtening ties with foreign research institutions. The control of particular outputs is not implemented by the evalution committee, but the correctness of allocated finances and the adequacy of their use are checked.

Realization period - beginning

Jan 1, 2013

Realization period - end

Dec 31, 2014

Project status

U - Finished project

Latest support payment

Feb 4, 2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data delivery code

CEP15-MSM-7A-U/01:1

Data delivery date

Jul 2, 2015

Total approved costs

170 thou. CZK

Public financial support

170 thou. CZK

Other public sources

0 thou. CZK

Non public and foreign sources

0 thou. CZK