Quantitative measurement and imaging of drug-uptake by bacteria with antimicrobial resistance
Public support
Provider
Ministry of Education, Youth and Sports
Programme
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Call for proposals
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Main participants
Český metrologický institut
Contest type
M2 - International cooperation
Contract ID
MSMT-21096/2016-7
Alternative language
Project name in Czech
Quantitative measurement and imaging of drug-uptake by bacteria with antimicrobial resistance
Annotation in Czech
1. To develop urgently needed new metrological capabilities for: the label-free 3D imaging of antibacterial agents in bacteria. This requires a new 3D chemical imaging instrument with 100 times better sensitivity and a high-spatial resolution (100 nm). The instrument will have a mass resolution of >100 000 and the ability to sample from sub-micron areas, simultaneously. the traceable quantification of the vertical concentration profile of antibacterial agents in bacteria and biofilms. Measurements will be performed in liquid and at near ambient pressure. imaging surface macromolecules, such as porins or metal-transport proteins, to study the efflux mechanisms in Gram-negative bacteria and to give real-time quantitative measurements of drug-uptake in bacteria and biofilms. Numerical modelling and algorithms will be developed to support measurements in complex biological environments. 2. To develop well-controlled model systems to allow cross-platform measurement of penetration, accumulation and efflux of antibacterial agents in single cells, in suspended cellular aggregates, as well as in biofilm communities including binding to biofilm matrix components. The efficacy of novel antibacterial agents and efflux pump inhibitors will be investigated. 3. To develop signal enhancement strategies and advanced sample preparation methods for studying antibacterial agents in bacteria and biofilms including: advanced cryo preparation methods to enable ‘liquid’ (vitrified) water to be present in the vacuum of high-performance metrology instruments without ultrastructural reorganisation and translocation of exo/endo -genous molecules.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EE - Microbiology, virology
CEP - secondary branch
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CEP - another secondary branch
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OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10606 - Microbiology<br>10607 - Virology
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
Following the condition that the candidate of financial contribution was evaluated and afterwards selected by international provider in accordance with the rules of the program the Ministry of Education, Youth ans Sports does not realize the evaluation of project results. The project is evaluated only after its approval by an international provider.
Solution timeline
Realization period - beginning
May 1, 2016
Realization period - end
Apr 30, 2019
Project status
U - Finished project
Latest support payment
Feb 12, 2019
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP20-MSM-8B-U/01:1
Data delivery date
Jun 18, 2020
Finance
Total approved costs
3,909 thou. CZK
Public financial support
2,050 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
1,859 thou. CZK