Protein modification by reactive aldehydes derived from polyamine metabolism
Public support
Provider
Ministry of Education, Youth and Sports
Programme
Programme for Funding Multilateral Scientific and Technological Cooperation Projects in the Danube Region
Call for proposals
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Main participants
Univerzita Palackého v Olomouci / Přírodovědecká fakulta
Contest type
M2 - International cooperation
Contract ID
-
Alternative language
Project name in Czech
Modifikace proteinů reaktivními aldehydy odvozenými z metabolismu polyaminů
Annotation in Czech
The aim of this study is to evaluate the impact of polyamine-derived aminoaldehydes on living cells, focusing on the induced protein modifications, their characteristics, and extent. We plan to treat model and real protein samples with the aminoaldehydes APAL, AcAPAL, and BOPBD. These compounds can be obtained commercially (APAL) or synthesized in the laboratory: AcAPAL via acetylation of APAL, and BOPBD either through enzymatic spermine oxidation using purified pea seedling diamine oxidase or by chemical oxidation of spermidine. These compounds will serve as reagents for experimental treatments. The model proteins will include serum albumin, hemoglobin, and other selected proteins including enzymes. Real protein samples will be derived from rat brain lysates and protein extracts from microorganisms, including bacteria (e.g., Escherichia coli), yeast (Saccharomyces cerevisiae), and cyanobacteria (Synechococcus sp.). Additionally, we will use hepatocytes and rat brain cells as protein sources. Microbial, hepatic, or rat brain cells will also be directly treated with the reactive compounds at different concentrations, and the resulting protein extracts or tissue samples will be analyzed.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10608 - Biochemistry and molecular biology
OECD FORD - secondary branch
10609 - Biochemical research methods
OECD FORD - another secondary branch
10406 - Analytical chemistry
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CB - Analytical chemistry, separation<br>CE - Biochemistry<br>EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jul 1, 2025
Realization period - end
Jun 30, 2027
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP25-MSM-8X-R
Data delivery date
Aug 6, 2025
Finance
Total approved costs
250 thou. CZK
Public financial support
250 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK