Generation of temperature sensitive cell division cycle mutants in green alga Chlamydomonas reinhardtii
Project goals
Cell cycle study has been one of the fastest evolving areas in today's biology. Most of what we know about the cell cycle regulation is thanks to the production of conditional cell division cycle mutants generated in 1970s by Leland Hartwell in Saccharomyces cerevisiae and Paul Nurse in Schizosaccharomyces pombe. Here, we propose to generate a temperature sensitive cell division cycle mutants in the green alga Chlamydomonas reinhardtii, a model plant single celled organism. The aims of the proposal are:1) produce temperature sensitive cell division cycle mutants of Chlamydomonas reinhardtii, 2) identify cell cycle phenotypes and execution points for the mutants, 3) form complementation groups, 4) characterize in more detail one to two of the most interesting mutants.
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 12 (SGA02009GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
204/09/0111
Alternative language
Project name in Czech
Příprava teplotně sensitivních mutantů buněčného cyklu u zelené řasy Chlamydomonas reinhardtii
Annotation in Czech
Cílem projektu je příprava teplotně sensitivních mutantů buněčného cyklu u jednobuněčného modelového organismu rostlinné říše, zelené řasy Chlamydomonas reinhardtii. U těchto mutantů stanovíme fenotyp, zařadíme je do komplementačních skupin a jeden či dva nejzajímavější podrobně charakterizujeme.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
—
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project was excellent, both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Mar 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GA-U/02:2
Data delivery date
May 2, 2016
Finance
Total approved costs
2,492 thou. CZK
Public financial support
2,492 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 492 CZK thou.
Public support
2 492 CZK thou.
100%
Provider
Czech Science Foundation
CEP
EB - Genetics and molecular biology
Solution period
01. 03. 2009 - 31. 12. 2011