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Development of chiral vibrational spectroscopy for solid state systems

Project goals

The spectroscopy of vibrational optical activity provides unique information about molecular structure, but its measurement and interpretation for crystals, precipitates and living cells is difficult. We aim to develop experimental and computational methodologies applicable to studies of aggregated proteins and crystals relevant to selected biological and medical applications. On a set of solid state compounds relevant to the needs of the pharmacological industry we will develop vibrational circular dichroism experiment to discriminate between various crystalline forms. For model protein fibrils we will elaborate Raman optical activity procedures to understand the peptide folding and precipitation. Finally, theoretical models and computational protocols will be found for the link between the spectra and the structure. The project combines unique knowledge of crystallographic and spectroscopic laboratories, includes other collaborations, the results will be applicable in analytical chemistry, biology and drug development.

Keywords

vibrational circular dichroismRaman optical activityelectron diffractioncrystal-structuresolid-statecomputational modeling

Public support

  • Provider

    Czech Science Foundation

  • Programme

    Standard projects

  • Call for proposals

    SGA0202400001

  • Main participants

    Ústav organické chemie a biochemie AV ČR, v. v. i.

  • Contest type

    VS - Public tender

  • Contract ID

    24-10558S

Alternative language

  • Project name in Czech

    Vývoj chirální vibrační spektroskopie pro systémy v tuhém stavu

  • Annotation in Czech

    Spektroskopie vibrační optické aktivity poskytuje unikátní informace o molekulové struktuře, ale její měření a interpretace pro krystaly, sraženiny a živé buňky je obtížné. Chceme tak vyvinout experimentální a teoretické postupy aplikovatelné pro výzkum agregovaných bílkovin a krystalů pro aplikace užitečné v biologii a medicíně. Na sérii látek v tuhém stavu vyvineme protokol pro měření vibračního cirkulárního dichroismu pro rozlišovaní různých krystalových struktur. Pro modelové bílkovinné fibrily vypracujeme měření Ramanovy optické aktivity pro výzkum konformací a srážení peptidů. Najdeme také teoretické a výpočetní postupy popisující vztah mezi spektry a strukturou. Projekt kombinuje unikátní zkušenosti krystalografické a spektroskopické laboratoře, zahrnuje další spolupráce, výsledky budou aplikovatelné v analytické chemii, biologii a pro vývoj léčiv.

Scientific branches

  • R&D category

    ZV - Basic research

  • OECD FORD - main branch

    10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

  • OECD FORD - secondary branch

    10306 - Optics (including laser optics and quantum optics)

  • OECD FORD - another secondary branch

    10403 - Physical chemistry

  • BE - Theoretical physics
    BH - Optics, masers and lasers
    CF - Physical chemistry and theoretical chemistry

Solution timeline

  • Realization period - beginning

    Jan 1, 2024

  • Realization period - end

    Dec 31, 2026

  • Project status

    B - Running multi-year project

  • Latest support payment

    Apr 9, 2024

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP25-GA0-GA-R

  • Data delivery date

    Feb 21, 2025

Finance

  • Total approved costs

    10,233 thou. CZK

  • Public financial support

    8,984 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    1,161 thou. CZK

Basic information

Recognised costs

10 233 CZK thou.

Public support

8 984 CZK thou.

87%


Provider

Czech Science Foundation

OECD FORD

Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Solution period

01. 01. 2024 - 31. 12. 2026