Architecture and evolution of protein synthesis apparatus in plastids
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Univerzita Karlova / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
26-20560S
Alternative language
Project name in Czech
Architecture and evolution of protein synthesis apparatus in plastids
Annotation in Czech
A plastid is an endosymbiotic organelle that has retained (reduced) genome and the machinery for its expression, including ribosomes (plastoribosomes). The structure of the plastoribosome has only been studied in a land plant (spinach). Here, we integrate various experimental and bioinformatic approaches to study the diversity of plastoribosomes across eukaryotes. Using cryo-EM, we will determine plastoribosome structures from three distantly related species, chosen for their evolutionary and socioeconomic significance. In two other species, we will identify plastoribosomal proteins through tagging and IP-MS or by spatial proteomics. Bioinformatic analyses will map plastoribosomal protein distributions across plastid-bearing groups, revealing evolutionary history, inter-ribosomal protein transfer, and past endosymbiotic events. Our findings will shed light on the evolution of the plastoribosome and complex plastids, enable comparisons with mitoribosomes, and may provide a basis for optimizing biotechnological production.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EA - Morphology and cytology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
May 21, 2026
Finance
Total approved costs
12,051 thou. CZK
Public financial support
12,051 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK