Identification of novel factors assisting and counteracting BRCA2 activity during meiotic DNA repair in Caenorhabditis elegans
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Masarykova univerzita / Lékařská fakulta
Contest type
VS - Public tender
Contract ID
26-20768S
Alternative language
Project name in Czech
Identification of novel factors assisting and counteracting BRCA2 activity during meiotic DNA repair in Caenorhabditis elegans
Annotation in Czech
Induction and repair of DNA breaks in meiosis ensures faithful chromosome segregation and maintenance of genome integrity. Homologous recombination is the preferred DNA repair system in the gametes and requires BRCA2-dependent loading of the recombinases RAD51-DMC1. Null BRCA2 mutations in mouse are embryonic lethal, which has hindered its study in the germ line, leaving its roles in meiosis poorly understood. We generated a functional CRISPR-tagged line of C. elegans brc-2/BRCA2 to isolate interactors in meiotic cells via proteomic approach. We identified the novel factor RIPR-1 and found that has essential roles during meiotic DNA repair by acting in a complex with BRC-2. Through genetics, cell biology and biochemical tools we will dissect the meiotic functions of RIPR-1 and its functional interplay with BRC-2 as well as with FIGL-1/FIGNL1, shown to counteract BRCA2 activity in other organisms, that has remained largely uncharacterized in worms. Our analysis will shed new light on the requirements imposed by RIPR-1-BRC-2-FIGL-1 functions during germ cells development in metazoans.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10603 - Genetics and heredity (medical genetics to be 3)
OECD FORD - secondary branch
10605 - Developmental biology
OECD FORD - another secondary branch
10608 - Biochemistry and molecular biology
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CE - Biochemistry<br>EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
May 4, 2026
Finance
Total approved costs
7,055 thou. CZK
Public financial support
6,977 thou. CZK
Other public sources
78 thou. CZK
Non public and foreign sources
0 thou. CZK