Ultraviolet Photodissociation Mass Spectrometry for Advanced Structural Analysis of Lipids
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Ústav organické chemie a biochemie AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-20832S
Alternative language
Project name in Czech
Ultraviolet Photodissociation Mass Spectrometry for Advanced Structural Analysis of Lipids
Annotation in Czech
Advances in lipid science have deepened our understanding of lipid functions across biological systems. Mass spectrometry (MS) plays a central role in lipid analysis, offering high sensitivity for identification and quantification. Tandem MS with collision-induced dissociation (CID) is widely used for structural analysis, but often fails to resolve critical features, especially in isomeric lipids. To address these limitations, alternative methods such as UV photodissociation (UVPD) are increasingly used. UVPD accesses distinct fragmentation pathways, opening new avenues for structural analysis, though its underlying mechanisms remain only partially understood. In our recent study, we observed photofragmentation of lithium-cationized wax esters, and proposed a mechanism involving Norrish–Yang-type photochemistry. This project will investigate the photofragmentation in detail, combining experimental and theoretical approaches. We will target lipid classes where CID is inadequate, aiming to show that UVPD-driven fragmentation enables structural insights beyond those offered by CID.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10403 - Physical chemistry
OECD FORD - secondary branch
10406 - Analytical chemistry
OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
CB - Analytical chemistry, separation<br>CF - Physical chemistry and theoretical chemistry
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
May 11, 2026
Finance
Total approved costs
10,676 thou. CZK
Public financial support
10,232 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
444 thou. CZK