Impact of mTOR signaling modulation in NG2-expressing cells on the neuroregeneration after ischemic brain injury
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Biotechnologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-21470S
Alternative language
Project name in Czech
Impact of mTOR signaling modulation in NG2-expressing cells on the neuroregeneration after ischemic brain injury
Annotation in Czech
The present project aims to evaluate the impact of mTOR signaling modulation on the proliferation and differentiation of NG2 glia, oligopotent cells involved in brain repair after ischemic stroke, and on their contribution to post-ischemic tissue regeneration. Our goal is to investigate the cellular and molecular mechanisms that may counteract damage induced by focal cerebral ischemia (FCI) and support neuroregeneration. Our preliminary data indicate that mTOR signaling is upregulated in NG2 glia in the ischemic mouse brain. Therefore, we will use transgenic animals to inhibit or activate mTOR signaling specifically in NG2 glia, in an animal model of FCI induced by permanent occlusion of the middle cerebral artery. The effect of mTOR signaling modulation on the properties of NG2 glia at the cellular level and in vivo will be evaluated by gene expression profiling, proteomics, functional analyses, and behavioral testing. Since no broadly applicable treatment for stroke exists, we believe that our findings may help identify new directions for glia-oriented therapeutic strategies.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
30103 - Neurosciences (including psychophysiology)
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
FH - Neurology, neuro-surgery, nuero-sciences
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
May 21, 2026
Finance
Total approved costs
13,995 thou. CZK
Public financial support
13,995 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK