Using Reverse Genetics in Amphioxus to Inform the Development and Evolution of Chordates
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Ústav molekulární genetiky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-22446S
Alternative language
Project name in Czech
Using Reverse Genetics in Amphioxus to Inform the Development and Evolution of Chordates
Annotation in Czech
As a representative of a basal lineage within the chordate phylum, amphioxus retains many characteristics thought to have been present in the common chordate ancestor. By studying amphioxus, we can thus infer how key vertebrate traits evolved over time. Most of the current insight into vertebrate evolution using amphioxus as a model is based on descriptive gene expression studies. Here, we plan to leverage the power of CRISPR/Cas9-based reverse genetics combined with phenotypic and molecular characterization of mutants. By inactivating evolutionarily conserved genes implicated in the development of the visual system and placodes we aim to provide fundamentally novel insight into the ancestral chordate state and vertebrate-specific innovations.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10605 - Developmental biology
OECD FORD - secondary branch
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OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EB - Genetics and molecular biology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
Apr 27, 2026
Finance
Total approved costs
8,865 thou. CZK
Public financial support
8,865 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK