Effect of synthetic cytokinin analogues on plant hormone perception, metabolism and phenotypic alterations
Project goals
Synthetic derivatives of plant hormones cytokinins tested so far in our laboratory affect cytokinin degradation by cytokinin dehydrogenase and/or cytokinin perception by receptors in vitro. The objective of proposed project is screening of developmentalchanges after application of selected cytokinin analogues to plants which will be accompanied by detailed study of expression of genes involved in cytokinin biosynthesis, perception and deactivation as well as by monitoring level of cytokinin metabolitesand auxins. Newly synthesized cytokinin analogues and compounds acting as inhibitors of cytokinin biosynthesis will be also tested. In addition, an alternative mechanism of cytokinin deactivation by xanthine dehydrogenase will be verified and physiological function of non-secreted cytokinin dehydrogenase AtCKX7 will be elucidated. Developmental changes triggered by different compounds can be used to study distinct metabolic pathways involved in cytokinin homeostasis and the compounds may find an application for improvement of yield attributes of agricultural crops.
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 11 (SGA02008GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
522/08/0920
Alternative language
Project name in Czech
Vliv syntetických derivátů cytokininů na percepci a metabolismus rostlinných hormonů a fenotypické změny
Annotation in Czech
Cílem projektu je studovat vývoj rostliny, expresi genů metabolismu cytokininů (CK) a hladiny CK a auxinů po aplikaci derivátů CK, testovat nová analoga CK a inhibitory biosyntézy, ověřit alternativní mechanismus deaktivace CK a zkoumat fyziologickou funkci nesekretované cytokinindehydrogenasy.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
ED - Physiology
CEP - secondary branch
CE - Biochemistry
CEP - another secondary branch
EB - Genetics and molecular biology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
30105 - Physiology (including cytology)
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project was excellent, both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GA-U/02:2
Data delivery date
May 2, 2016
Finance
Total approved costs
5,498 thou. CZK
Public financial support
5,498 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
5 498 CZK thou.
Public support
5 498 CZK thou.
100%
Provider
Czech Science Foundation
CEP
ED - Physiology
Solution period
01. 01. 2008 - 31. 12. 2011