The role of Akt kinase in the regulation of G2/M cell cycle transition and in the acquisition of developmental potency of oocytes
Project goals
In the proposed project regulation of G2/M checkpoint of meiotic cell cycle will be addressed in order to assess the relevance of these events for improvement of oocyte quality and developmental potency of early embryos. The data issued from this studywill also contribute to the understanding of cell cycle regulation. For this purpose the role of Akt kinase, an upstream regulator of p34cdc2 kinase, will be analysed during meiosis progression. To achieve this goal the experimental model of in vitromaturation of mouse, porcine and cattle oocytes will be used . Detailed analysis of expression, activation and localisation of Akt kinase and downstream Wee1 kinase family during meiosis progression will be performed. Dynamics of p34cdc2 kinaseactivation willbe documented as well. Subsequently, specific inhibitors of Akt kinase activity will be used during in vitro culture in order to affect cytoplasmic maturation and to improve quality of oocytes. The impact of inhibition of Akt kinase upon
Keywords
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 2 (SGA02003GA-ST)
Main participants
Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Úloha Akt kinázy pro regulaci G2/M fáze buněčného cyklu a získání vývojové kompetence oocytů
Annotation in Czech
Navrhovaný projekt se zabývá regulací G2/M fáze meiotického buněčného cyklu s cílem využít tyto poznatky pro zvýšení kvality zralých oocytů a vývojového potenciálu časných embryí. Údaje získané při řešení tohoto projektu také přispějí k poznání regulacebuněčného cyklu. Za tímto účelem bude v průběhu meiotického zrání analyzována úloha kinázy Akt (protein kináza B), která reguluje aktivitu p34cdc2 kinázy. Pro řešení tohoto úkolu bude používán in vitro model meiotického zrání oocytů myši, prasete askotu.V závislosti na jednotlivých stádiích meiózy bude provedena podrobná analýza exprese, aktivace a lokalizace Akt kinázy a jí podřízených Wee1 kináz, jakož i bude podrobně dokumentována dynamika aktivity p34cdc2 kinázy. V návazných experimentechbudou při kultivaci oocytů použity specifické inhibitory Akt kinázy s cílem ovlivnit cytoplasmatické zrání a zvýšit kvalitu oocytů. Bude provedena morfologická a biochemická analýza účinku inhibice kinázy Akt v průběhu meiotického zrání oocytů myši,
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
ED - Physiology
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
GI - Farm animal breeding and farm animal pedigree breeding
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30105 - Physiology (including cytology)
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40203 - Husbandry
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
Phosphorylation and activation of protein kinase Akt (protein kinase B) was evaluated during meiotic maturation of mouse and porcine oocytes. At germinal vesicle breakdown (GVBD) of mouse oocytes the phosphorylation and activity of Akt/PKB was increased
Solution timeline
Realization period - beginning
Jan 1, 2003
Realization period - end
Jan 1, 2005
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP06-GA0-GA-U/07:6
Data delivery date
Jan 15, 2009
Finance
Total approved costs
2,745 thou. CZK
Public financial support
1,860 thou. CZK
Other public sources
885 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
2 745 CZK thou.
Public support
1 860 CZK thou.
67%
Provider
Czech Science Foundation
CEP
ED - Physiology
Solution period
01. 01. 2003 - 01. 01. 2005