Molecular basis of osmotolerant yeast Z. rouxii, Z. bailii and D. hansenii specific properties and their biotechnological applications
Project goals
Aim of this project is to characterize specific properties of the non-conventional osmotolerant yeast species Debaryomyces hansenii, Zygosaccharomyces rouxii and Zygosaccharomyces bailii at molecular level in order to contribute to the improvement of industrial Saccharomyces cerevisiae strains. The project will consist of three parts: (1) isolation of new genes involved in fructose, glycerol and alkali-metal-cation transport together with the characterization of their products, (2) development of new tools for genome engineering of Z. bailii and D. hansenii, and (3) construction and phenotypic characterization of new S. cerevisiae strains with improved growth/tolerance capacities by heterologous (over)expression of the isolated genes from non-conventional yeast species.
Keywords
osmotolerantyeastsugartransportglyceroltransportcationhomeostasistoolsgeneticengineering
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
Standardní projekty 13 (SGA02010GA-ST)
Main participants
—
Contest type
VS - Public tender
Contract ID
P503-10-0307
Alternative language
Project name in Czech
Molekulární podstata specifických vlastností osmotolerantních kvasinek Z. rouxii, Z. bailii a D. hansenii a jejich biotechnologické aplikace
Annotation in Czech
Cílem projektu je charakterizovat specifické rysy nekonvenčních osmotolerantních kvasinek Debaryomyces hansenii, Zygosaccharomyces rouxii a Zygosaccharomyces bailii na molekulární úrovni a přispět tak ke zlepšení vlastností průmyslových kmenů Saccharomyces cerevisiae. Projekt se skládá ze tří částí: (1) izolace dosud neznámých genů důležitých pro transport fruktosy, glycerolu a kationů alkalických kovů a charakterizace jejich produktů, (2) vytvoření nových nástrojů genového inženýrství pro Z. bailii a D. hansenii, a 3) konstrukce a fenotypová charakterizace nových kmenů S. cerevisiae se zlepšenými růstovými/tolerančními vlastnostmi pomocí heterologní exprese genů izolovaných z nekonvenčních druhů kvasinek.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EE - Microbiology, virology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30101 - Human genetics
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project brought original information about the fructose, glycerol and potassium transporters in osmotolerant yeast species, and their possible use in biotechnology. The results were largely published or submitted for publication in impacted journals.No problems were found either in maintaining the grant rules or using the financial means.
Solution timeline
Realization period - beginning
Jan 1, 2010
Realization period - end
Dec 31, 2014
Project status
U - Finished project
Latest support payment
Mar 19, 2014
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP15-GA0-GA-U/01:1
Data delivery date
May 22, 2015
Finance
Total approved costs
5,551 thou. CZK
Public financial support
5,351 thou. CZK
Other public sources
200 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
5 551 CZK thou.
Public support
5 351 CZK thou.
96%
Provider
Czech Science Foundation
CEP
EI - Biotechnology and bionics
Solution period
01. 01. 2010 - 31. 12. 2014