Mechanism of ergosterol - plant cell interaction
Project goals
Lipid substances are responsible for many signal mechanisms in plants. They play the role of hormones (brassinosteroids) or signal molecules (ergosterol). Ergosterol, the main sterol of the fungi cytoplasmatic membrane, is one of the lipids which activate defense reaction in plants; at standard conditions it is not present in them. It triggers defense reaction in tobacco cells even in nanomolar concentration. The defense reaction is characterized by production of active oxygen species, alkalization ofextracellular medium, production of phytoalexin capsidiol and activation of pathogen related proteins expression, on the other hand, it does not lead to cell death. Thus ergosterol is suitable for protecting plants against fungi. However, the mechanism of triggering the plant defense reaction by ergosterol is not known for the present. The project will be focused on early recognition of ergosterol by tobacco cells. Moreover, the signal pathway elicited by ergosterol has not been fully explained yet. Bynow it has been found that ergosterol triggers defense reaction via activation of A2 phospholipase and C proteinkinase. Therefore, the project will be also focused on completion of the so far unexplained components of the signal pathway elicited by ergosterol (e g. release of Ca2+ from a vacuole).
Keywords
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 9 (SGA02009GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
203/09/P248
Alternative language
Project name in Czech
Mechanizmus interakce ergosterolu s rostlinnou buňkou
Annotation in Czech
1) Studium mechanizmu rozpoznání ergosterolu buňkami tabáku. 2) Studium interakce lipidických signálních molekul zapojených do signální dráhy vyvolané ergosterolem (lysophospolipidy, mastné kyseliny) s membránou vakuoly.
Scientific branches
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project gone according to plan both in terms of expertise and in terms of disbursement of funds.
Solution timeline
Realization period - beginning
Jan 1, 2009
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Apr 16, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-GA0-GP-U/03:3
Data delivery date
May 2, 2016
Finance
Total approved costs
1,305 thou. CZK
Public financial support
1,305 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
1 305 CZK thou.
Public support
1 305 CZK thou.
0%
Provider
Czech Science Foundation
CEP
CE - Biochemistry
Solution period
01. 01. 2009 - 31. 12. 2011