Functional analysis of methyltransferase involved in SL RNA modification in Trypanosoma brucei
Project goals
The trypanosomatid SL RNA plays an essential role in trans-splicing and processing of all mRNA. SL RNA provides the m7G-capped SL sequence to the 5'end of every mRNA. The cap structure of the SL RNA is unique in eukaryotes with 4 nucleotides after the m7G cap carrying a total of seven methyl groups and by convention is referred to as "cap 4". The RNA methyltransferases that catalyze cap 4 modifications are unique to trypanosomatids. It is likely, that the enzymes involved in cap 4 formation, play a pivotal role in regulating the availability of trans-splicing "competent" SL RNA and, therefore, these enzymes might be major players in regulating trans-splicing activity as a whole. Recently, several cap 4 modifying enzymes have been characterized. Theaimof this project is to analyze the function of potential SL RNA methyltransferase MT420 in Trypanosoma brucei. The specific aims of the project involve investigation of a role of MT420 for SL RNA biogenesis and trans-splicing, determination its
Keywords
T. bruceimethyltranserasesSL RNAtrans-splicingcap structureRNA interference
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 8 (SGA02008GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
204/08/P585
Alternative language
Project name in Czech
Funkční analýza methyltransferázy účastnící se modifikace SL RNA u Trypanosoma brucei
Annotation in Czech
SL RNA u trypanosomatid má významnou roli při "trans-sestřihu" a úpravách mRNA. SL RNA poskytuje každé mRNA tzv. SL sekvenci. SL sekvence je krátký úsek SL RNA charakterizovaný m7G čepičkou na 5´-konci následovanou 4 nukleotidy nesoucími sedm methylových skupin. Tato SL RNA čepička je označována jako "cap 4" a je mezi eukaryotickými organismy jedinečná. RNA methyltransferázy, které katalyzují modifikace SL sekvence u trypanosom jsou rovněž unikátní. Je pravděpodobné, že tyto enzymy, účastnícíse modifikace SL sekvence, hrají hlavní roli v regulaci dostupnosti SL RNA pro "trans-sestřih" a mohou tak být důležitými aktéry v regulaci tohoto zásadního procesu. Během posledních let bylo charakterizováno několik enzymů účastnících se modifikace čepičky SL RNA. Cílem tohoto projektu je analyzovat funkci potenciální methyltransferázy MT420 účastnící se modifikace SL sekvence u Trypanosoma brucei - významného parazitického prvoka savců.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
CE - Biochemistry
CEP - another secondary branch
—
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
Completed project evaluation
Provider evaluation
O - Nesplněno zadání, smlouva však byla dodržena
Project results evaluation
This project has focused on the characterization of the putative methyltransferase MT420 in Trypanosoma brucei, a human pathogenic parasite. MT420 is annotated as a hypothetical protein and was originally thought to play a role in the methylation of theunique cap structure of all T. brucei mRNAs. However, this hypothesis was shown to be incorrect. Bioinformatics have allowed us to identify hig
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Dec 31, 2010
Project status
U - Finished project
Latest support payment
Apr 16, 2010
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP11-GA0-GP-U/04:3
Data delivery date
Mar 20, 2015
Finance
Total approved costs
1,035 thou. CZK
Public financial support
1,035 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
1 035 CZK thou.
Public support
1 035 CZK thou.
100%
Provider
Czech Science Foundation
CEP
EB - Genetics and molecular biology
Solution period
01. 01. 2008 - 31. 12. 2010