Development of in vitro transcription system for Corynebacterium glutamicum and characterization of RNA polymerase activity
Public support
Provider
Czech Science Foundation
Programme
Post-graduate (doctorate) grants
Call for proposals
Postdoktorandské granty 12 (SGA02012GA1PD)
Main participants
—
Contest type
VS - Public tender
Contract ID
P302-12-P633
Alternative language
Project name in Czech
Vývoj systému in vitro transkripce pro Corynebacterium glutamicum a charakterizace aktivity RNA polymerasy
Annotation in Czech
Transkripce in vitro je účinným nástrojem analýzy regulace genové exprese u bakterií. Pro studium významného producenta aminokyselin Corynebacterium glutamicum nebyl dosud systém transkripce in vitro k dispozici. V předběžných experimentech jsme RNA polymerasu (RNAP) z C. glutamicum izolovali a prokázali její funkci in vitro. V předkládaném projektu bude plně aktivní holoenzym RNAP, připravený rekonstitucí purifikovaného jádra RNAP a sigma podjednotek, tvořit základ spolehlivého systému transkripce in vitro pro C. glutamicum. Různé kombinace jádra RNA polymerasy a jednotlivých sigma faktorů umožní identifikaci sigma faktorů účastnících se transkripce ze studovaných promotorů C. glutamicum. Optimalizovaný systém transkripce in vitro bude použit při analýze některých vlastností RNAP z C. glutamicum a pro jejich porovnání s vlastnostmi RNA polymeras z Bacillus subtilis a Escherichia coli.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EE - Microbiology, virology
CEP - secondary branch
EB - Genetics and molecular biology
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The in vitro transcription system for Corynebacterium glutamicum was developed and exploited for the study of sigma factor specificity. The system became the basis for newly developed ROSE technique for analysis of promotors on genome level. The projectoutput consists of two high quality papers and one book chapter. Obtained results are significant for understanding of transcription regulation.
Solution timeline
Realization period - beginning
Jan 1, 2012
Realization period - end
Dec 31, 2014
Project status
U - Finished project
Latest support payment
Mar 31, 2014
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP15-GA0-GP-U/02:2
Data delivery date
May 6, 2016
Finance
Total approved costs
2,430 thou. CZK
Public financial support
2,430 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK