Scaffolds from liposome nanofibers for tissue engineering
Project goals
Cartilages breakdown due to disease results in a severe pain and disability. A new approach has recently appeared: implantation of the autologous chondrocytes and construction of artificial cartilages based on seeding of autologous chondrocytes on biodegradable artificial scaffolds. Our preliminary studies suppose to finish preparation of several types of suitable composite biodegradable scaffolds based on non-woven nanofibres in combination with liposomes. The main goal of this proposal is to develop the nanofibrous scaffolds with various liposomes in nanofibres, and to control their opening with ultrasound wavelengths. Our proposal is concentrated on: 1. Development of liposome nanofibres and methods for liposome opening both in vitro and in vivo 2.Preparation of biodegradable scaffolds from liposome nanofibres for tissue engineering 3. Production of artificial cartilage from autologous chondrocytes based on new types of scaffolds
Keywords
scaffoldsfibrous materialsnanofiberselectrospinningliposomeschondrocytesdrug deliveryartificial tissue
Public support
Provider
Academy of Sciences of the Czech Republic
Programme
Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic
Call for proposals
Výzkumné granty 7 (SAV02007-A)
Main participants
—
Contest type
VS - Public tender
Contract ID
IAA500390702
Alternative language
Project name in Czech
Tkáňové nosiče z nanovlákenných materiálů s vestavěnými liposomy
Annotation in Czech
Poškození chrupavek vede v konečném důsledku k velmi bolestivým stavům a invaliditě. V nedávné době se objevil nový přístup k léčbě: implantace autologních chondrocytů a konstrukce artificiálních chrupavek. V tomto přístupu se využívá biodegradabilních nosičů a jejich následného osídlení autologními chondrocyty. Naše předběžné výsledky ukazují, že jsme schopni vytvořit biodegradabilní nosič na bázi netkaných nanovláken v kombinaci s liposomy. Hlavním cílem tohoto návrhu je vytvořit nanovlákenný nosič srůznými druhy liposomů a vytvořit metodiku jejich postupného otevírání s využitím ultrazvukových vln. Náš návrh se tedy soustřeďuje na: 1. Vývoj liposomových nanovláken a metodiky otevírání liposomů jak in vitro, tak i in vivo 2. Přípravu biodegradabilního nosiče liposomových nanovláken pro tkáňové inženýrství 3. Přípravu artificiálních chrupavek z autologních chondrocytů na bázi nově vyvinutých nosičů.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
BO - Biophysics
CEP - secondary branch
EI - Biotechnology and bionics
CEP - another secondary branch
JI - Composite materials
10610 - Biophysics
20505 - Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics; filled composites)
20801 - Environmental biotechnology
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
20803 - Environmental biotechnology related ethics
20901 - Industrial biotechnology
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
30401 - Health-related biotechnology
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
30404 - Biomaterials (as related to medical implants, devices, sensors)
30405 - Medical biotechnology related ethics
40401 - Agricultural biotechnology and food biotechnology
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Functionalized scaffold based on nanofibers and blood derivatives, developed a novel method for description of diffusion and interaction of macromolecules with nanofibers and prepared water-soluble coaxial nanofibers was prepared.
Solution timeline
Realization period - beginning
Jan 1, 2007
Realization period - end
Dec 31, 2011
Project status
U - Finished project
Latest support payment
Mar 18, 2011
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP12-AV0-IA-U/02:2
Data delivery date
Jun 28, 2013
Finance
Total approved costs
4,391 thou. CZK
Public financial support
4,391 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Recognised costs
4 391 CZK thou.
Public support
4 391 CZK thou.
0%
Provider
Academy of Sciences of the Czech Republic
CEP
BO - Biophysics
Solution period
01. 01. 2007 - 31. 12. 2011