Genetics and Improvement of Industrial Microorganisms
Public support
Provider
Academy of Sciences of the Czech Republic
Programme
Scheme of targeted research and development support
Call for proposals
SAV0-SS2000
Main participants
Mikrobiologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
—
Alternative language
Project name in Czech
Genetika a šlechtění průmyslových mikroorganismů
Annotation in Czech
Jedná se o přípravu nových linkosamidových antibiotik u Streptomyces lincolnensis, S. roseolus a S. caelestis pomocí mutageneze a selekce a pomocí genového inženýrství. Nová antibiotika nebo jejich deriváty budou izolovány a jejich chemická struktura bude určena pomocí nukleární magnetické rezonance a hmotové spektrometrie. Kultivační podmínky pro produkci budou optimalizovány nejprve v laboratorním měřítku a v malých fermentorech. Získané výsledku budou použity v poloprovozním měřítku. Budou izovolánataková množství látek, aby mohla být testována jejich biologická aktivita. Klinické kmeny methicilin-resistentní bakterie Staphylococcus aureus budou použity jako testovací mikroorganizmy. Cílem projektu je získat nové antibakteriální látky proti rezistetním klinickým patogenním bakteriím.
Scientific branches
R&D category
NV - Nonindustrial research (Applied research excluded Industrial research)
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
EI - Biotechnology and bionics
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Some genes coding biosynthesis of the important antibiotic lincomycin were identified and genes and proteins with a regulatory role in biosynthesis were tested. Production Streptomyces lincolnensis strains and new lincomycin derivatives were prepared.
Solution timeline
Realization period - beginning
Jan 1, 2000
Realization period - end
Jan 1, 2004
Project status
U - Finished project
Latest support payment
—
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP/2005/AV0/AV05IB/U/N/2:1
Data delivery date
Jun 17, 2005
Finance
Total approved costs
11,099 thou. CZK
Public financial support
6,081 thou. CZK
Other public sources
5,018 thou. CZK
Non public and foreign sources
0 thou. CZK