Production of Dendrobium candidum biomass in vitro and study its biological activity
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT II
Call for proposals
KONTAKT II 2 (SMSM2012LH2)
Main participants
—
Contest type
VS - Public tender
Contract ID
MSMT-7026/2012-36
Alternative language
Project name in Czech
In vitro produkce biomasy Dendrobium candidum a studium její biologické aktivity
Annotation in Czech
Cíle projektu jsou následující: a) vypěstovat kalusovou a supsenzní kulturu Dendrobium candidum b) u vyprodukované biomasy ověřit protizánětlivou (inhibici cyklooxygenázy-1 a -2 a 5-lipoxygenázy) a antioxidační aktivitu (DPPH a ORAC metoda) c) identifikovat látky které jsou produkovány kalusovými nebo suspenzními kulturami d) ověřit možnosti zvýšení produkce protizánětlivých látek a antioxidantů v kalusových nebo suspenzních kulturách e) vznik vědecké spolupráce a výměny zkušeností mezi pracovišti ČLR aČR Tento projekt představuje, vzhledem ke své omezené délce, jen úvodní krok další spolupráce v této oblasti.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
—
CEP - another secondary branch
—
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
For one of tested stilbenes ? pinosltibene - strong cyklooxygenase-2 (COX-2) a cyklooxygenase-1 (COX-1) with IC50 values lower than reference inhibitors ibuprofen and indomethacin were recorded. Only pterostilben a oxyresveratrol were capable to inhibitcatalytic activity of 5-LOX with mild activity.
Solution timeline
Realization period - beginning
Mar 1, 2012
Realization period - end
Dec 31, 2013
Project status
U - Finished project
Latest support payment
Feb 27, 2013
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP14-MSM-LH-U/01:1
Data delivery date
Jun 30, 2014
Finance
Total approved costs
1,440 thou. CZK
Public financial support
1,440 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK