Involvement of nuclear phosphoinositides containing structures in gene expression
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Ústav molekulární genetiky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-13
Alternative language
Project name in Czech
Involvement of nuclear phosphoinositides containing structures in gene expression
Annotation in Czech
1) To develop experimental pipeline for isolation of PIPs-containing nuclear nanoparticles and analysis of their composition. 2) To determine the physico-chemical properties of isolated nanoparticles and analyze their function. 3) To produce artificial PIPs containing particles mimicking properties of Nuclear Lipid Islets and track their intranuclear dynamics. 4) To elaborate the contribution of phase separation to the dynamics and function of Nuclear Lipid Islets in chromatin remodeling and gene expression
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10601 - Cell biology
OECD FORD - secondary branch
10610 - Biophysics
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
BO - Biophysics<br>EA - Morphology and cytology
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
In the course of this project, we developed and optimized approaches for the isolation of lipid structures followed by mass spectrometry analysis. These and other developed procedures helped us to expand our knowledge about the nuclear PIPs structures. Making these procedures available to the wider European scientific community will enable their use in basic and clinical research.
Solution timeline
Realization period - beginning
May 15, 2020
Realization period - end
May 12, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
8,816 thou. CZK
Public financial support
8,816 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK