The application of CRISPR-Cas9 for the creation of multiple mutants in the genes coding for the nascent polypeptide associated complex
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Ústav experimentální botaniky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-24
Alternative language
Project name in Czech
The application of CRISPR-Cas9 for the creation of multiple mutants in the genes coding for the nascent polypeptide associated complex
Annotation in Czech
Flower and silique developments represent important processes of plant reproduction. Conventional Arabidopsis thaliana double homozygous mutant in the genes coding for the β-subunit of the nascent polypeptide associated complex (that were acquired by the cross of the available T-DNA insertion lines) showed notable phenotypic defects in the flowers and siliques. Moreover, seeds of the double homozygous mutant showed a reduced germination efficiency under the salt and osmotic stress. The aim of this project is to employ modern genome editing techniques to acquire the multiple mutants in the genes coding for the subunits of the nascent polypeptide-associated complex (a quintuple mutant of genes encoding its α-subunit and a double mutant in genes encoding the β-subunit). These genome-edited plants will be characterized in more detail and will elucidate the role of nascent polypeptide-associated complex during flower and silique developments of Arabidopsis thaliana. The key results of this project will include (1) the creation of various double mutants in the β-subunit of nascent polypeptide-associated complex and various quintuple mutants in the α-subunit of nascent polypeptide-associated complex by the modern genome-editing techniques (CRISPR-Cas9), (2) the characterization of various genome-editing events and phenotypic characterization of the plants with edited genomic DNA of the genes encoding the nascent polypeptide-associated complex, (3) the comparison of the genome-edited plants with the conventional double homozygous mutants of the β-subunit acquired by the cross of the available T-DNA insertion lines, (4) the creation of the plants with organ-specific genome editing by CRISPR-TSKO.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10611 - Plant sciences, botany
OECD FORD - secondary branch
—
OECD FORD - another secondary branch
—
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EF - Botany
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The aim of the project was to create a quintuple mutant in genes encoding for the α-subunit of the nascent polypeptide-associated complex (NAC) and a double mutant in genes encoding for NACβ by CRISPR-Cas9 technology. Both mutants were notably delayed in their development, and the nacα mutants showed also less chlorophyll in their leaves but the other photosynthetic parameters were not influenced.
Solution timeline
Realization period - beginning
Jun 1, 2020
Realization period - end
Mar 31, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
4,863 thou. CZK
Public financial support
4,863 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK