Glycan-dependent regulation of NK cell recognition: structural biology and novel methods of glycopeptide analysis
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Univerzita Karlova / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-42
Alternative language
Project name in Czech
Glycan-dependent regulation of NK cell recognition: structural biology and novel methods of glycopeptide analysis
Annotation in Czech
Project aims: Structure and glycan-dependent binding of selected human natural killer (NK) cell receptors to their newly described ligands will be studied by means of integrative structural biology and biochemistry. Systematic study of HILIC retention mechanisms of glycopeptides will be conducted and used to optimize mass spectrometry identification of isobaric glycopeptides of model and clinical samples.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10608 - Biochemistry and molecular biology
OECD FORD - secondary branch
10610 - Biophysics
OECD FORD - another secondary branch
30102 - Immunology
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
BO - Biophysics<br>CE - Biochemistry<br>EB - Genetics and molecular biology<br>EC - Immunology
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
In 2022 research from the 3rd project stage was performed according to the plan. Biophysical and structural characterization of the CD69:Gal-1, NKp30:B7-H6, and NKp46:Epa1 interactions related to N-glycosylation of the receptors continued. Results were presented within the INNOGLY COST Action and at international meetings; 2 publications were published, and 3 other manuscripts are in preparation.
Solution timeline
Realization period - beginning
May 15, 2020
Realization period - end
May 7, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
3,998 thou. CZK
Public financial support
3,998 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK