Diagnostic methods and control measures of viruses causing dwarf diseases and their vectors in cereal crops.
Project goals
The molecular methods of diagnosis and genetic diversity of virus diseases in cereal crops, the ecology of their vectors and protection measures against them will be investigated in the proposed project. The research will focus on: 1) the development andadaptation of molecular methods for reliable detection and identification of genetic diversity of Wheat dwarf virus (WDV), Barley yellow dwarf viruses (BYDVs), Barley mild mosaic virus (BaMMV) and Barley yellow mosaic virus (BaYMV), 2) the ecology, abundance and control measures against vectors, leafhopper Psammotettix alienus (for WDV)and aphids (for BYDVs), 3) the identification of resistance sources in wild species of Triticeae against WDV, 4) the evaluation of resistance status in winter wheat andbarley cultivars against WDV, 5) the assessment of resistance status in cultivars and breeding lines of wheat/barley/oat against BYDV strain MAV, and 6) the identification of Czech isolates of Polymyxa graminis and their transmission efficiency of BaMMV
Keywords
Cereal virusesreliable virus detectionvirus strainone-step RT-PCRreal-time PCRvirus vectorsprotection measuressource of resistenceTriticeaeAegilops
Public support
Provider
Ministry of Agriculture
Programme
The Research Program in the Agricultural Sector 2007 - 2012
Call for proposals
VaV pro zemědělský sektor 2 (SMZE2008MZE01)
Main participants
—
Contest type
VS - Public tender
Contract ID
45563/07-15010
Alternative language
Project name in Czech
Inovace diagnostických metod a ochranných opatření vůči virovým zakrslostem obilnin a jejich vektorům
Annotation in Czech
Vypracovat spolehlivé a citlivé metody molekulární diagnostiky hospodářsky významných virů obilnin. Objasnit ekologii přenašečů těchto virů a navrhnout systém ochrany vůči nim. Zjistit zdroje rezistence a stanovit odolnost odrůd obilnin vůči těmto virům.
Scientific branches
R&D category
AP - Applied research
CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
EB - Genetics and molecular biology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10606 - Microbiology
10607 - Virology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The project aim was to prepare reliable and sensitive methods of molecular diagnostics of economically significant viruses of cereals. To clarify the ecology of carriers of these viruses and propose a protection system against them. To find resistance sources and designate the resistance of varieties of cereals against these viruses. Individual objectives were fulfilled.
Solution timeline
Realization period - beginning
Jan 1, 2008
Realization period - end
Dec 31, 2012
Project status
U - Finished project
Latest support payment
Mar 2, 2012
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP13-MZE-QH-U/02:2
Data delivery date
Oct 16, 2013
Finance
Total approved costs
9,528 thou. CZK
Public financial support
9,045 thou. CZK
Other public sources
66 thou. CZK
Non public and foreign sources
417 thou. CZK
Basic information
Recognised costs
9 528 CZK thou.
Public support
9 045 CZK thou.
94%
Provider
Ministry of Agriculture
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
01. 01. 2008 - 31. 12. 2012