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New generations of DNA aptamers

Public support

  • Provider

    Technology Agency of the Czech Republic

  • Programme

    Programme of applied research and experimental development ALFA

  • Call for proposals

    ALFA 1 (STA02011TA01)

  • Main participants

    Ústav molekulární genetiky AV ČR, v. v. i.

  • Contest type

    VS - Public tender

  • Contract ID

    20110029

Alternative language

  • Project name in Czech

    Nové generace DNA aptamerů

  • Annotation in Czech

    Cílem tohoto projektu je navrhnout nové technologie přípravy oligonukleotidových DNA aptamerů a připravit aptamery, které by mohly být využitelné (po finálním dopracování) ke komerčním účelům. Konkrétně se jedná o aptamery využitelné pro izolaci teplotně-rezistentní Taq DNA polymerázy, pro hot-start PCR, pro identifikaci konzervativních komponent cytoskeletu (neurofilamenta a beta III tubulin) a malých molekul. V oboru malých organických molekul budou konkrétní látky (vyjmenované v části 2.3.) využíványpouze jako modely, cílem je vývoj univerzální technologie pro detekci těchto látek pomocí aptamerů ve velmi jednoduchém uspořádání, umožňujícím také variabilní imobilizaci rozpoznávací molekuly a tím sestavení víceúčelového čipu. Takto uspořádané testovací soupravy nepoužívající protilátky budou použitelné ke komerčním účelům.

Scientific branches

  • R&D category

    AP - Applied research

  • CEP classification - main branch

    EB - Genetics and molecular biology

  • CEP - secondary branch

    EI - Biotechnology and bionics

  • CEP - another secondary branch

    EB - Genetics and molecular biology

  • OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)

    10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30101 - Human genetics<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    The Commission notes that the results have been achieved in accordance with the Treaty / Appendix. According to the presentation, one of the results is ready for commercial use. The contract on the use of results is aimed at the use of other results.

Solution timeline

  • Realization period - beginning

    Jan 1, 2011

  • Realization period - end

    Dec 31, 2013

  • Project status

    U - Finished project

  • Latest support payment

    Mar 22, 2012

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP14-TA0-TA-U/03:1

  • Data delivery date

    Jul 3, 2020

Finance

  • Total approved costs

    13,690 thou. CZK

  • Public financial support

    10,900 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    2,790 thou. CZK