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Application of fluorescence methods to estimate the role of potassium transporters in the regulation of membrane potential and intracellular pH of yeast cells (KJB500110701)
and intracellular pH. The mail goal of this project is establishing a new method of fluorescence spectroscopy of cellular pH and membrane potential, using the diS-C3(3) fluorescence probe and a <...
EB - Genetika a molekulární biologie
- 2007 - 2009 •
- 1 349 tis. Kč •
- 1 349 tis. Kč •
- AV ČR
Řešení projektu: 1. 1. 2007 - 31. 12. 2009
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Akademie věd České republiky
MDR pumps in yeast kinetics, inhibition and the role of various physiological conditions;Dis-C3(3) fluorescence assay (GP202/04/P110)
The project is based on further development of a unique and well-established physical method for measurements of redistribution of the potentiometric fluorescent probe diS-C3(3) across biological membranes...
BG - Jaderná, atomová a molekulová fyzika, urychlovače
- 2004 - 2006 •
- 628 tis. Kč •
- 628 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 2004 - 1. 1. 2006
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Grantová agentura České republiky
Fluorescent techniques for the assassment of membrane potential in living cells (GA202/97/1121)
in living cells, which will be based on the use of fluorescent probes in combination biophysical techniques in both the selective detection of fluorescence emitted by probe respective fluorescence signals...
BG - Jaderná, atomová a molekulová fyzika, urychlovače
- 1997 - 1999 •
- 2 883 tis. Kč •
- 2 268 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 1997 - 1. 1. 1999
Uznané náklady
Podpora ze státního rozpočtu (79%)
Poskytovatel: Grantová agentura České republiky
Azaphthalocyanines – dark quenchers in DNA hybridization probes (GA17-19094S)
of the effect of labeling of ODN probe by two quenchers (3-end and intrastrand labeled ODN for DNA hybridization assays in double-labeled probes. The AzaPc quenchers are suitable for quenching of fluorescence of a...
CC - Organická chemie
- 2017 - 2021 •
- 5 890 tis. Kč •
- 5 890 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 2017 - 31. 12. 2021
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Grantová agentura České republiky
Design of fluorescence-based analytical tool for quantification of the protein-protein interaction within the 14-3-3 family (7AMB16DE005)
Aims: (1) to prepare a highly pure fluorescently labeled 14-3-3zeta WT protein; (2) to perform an NMR backbone assignment of the 14-3-3 monomeric variant; (3) to determine dimerization dissociation constan...
BO - Biofyzika
- 2016 - 2017 •
- 200 tis. Kč •
- 200 tis. Kč •
- MŠMT
Řešení projektu: 1. 1. 2016 - 31. 12. 2017
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Ministerstvo školství, mládeže a tělovýchovy
Molecular Fluorescent Probes for Selective Detection of Adenosine‐5’‐Triphosphate (ATP) (8J19UA048)
Elaboration of molecular fluorescent probes and test systems on their basis for selective detection of adenosine-5’-triphosphate (ATP) in aqueous solutions, and their following applications in pharmacology (as a method of preclinica...
Organic chemistry
- 2019 - 2020 •
- 92 tis. Kč •
- 92 tis. Kč •
- MŠMT
Řešení projektu: 1. 1. 2019 - 31. 12. 2020
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Ministerstvo školství, mládeže a tělovýchovy
Measurement of pH in model systems of liposomes by means of fluorescent probes using steady-state and time-resolved fluorescence techniques (GA202/99/0186)
in liposomes by means of fluorescent probes, including their application to the study fluorescence studies in both pure and protein-loaded liposomes stained by selected fluorescent dyes will provide better insight...
BG - Jaderná, atomová a molekulová fyzika, urychlovače
- 1999 - 2001 •
- 2 262 tis. Kč •
- 1 982 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 1999 - 1. 1. 2001
Uznané náklady
Podpora ze státního rozpočtu (88%)
Poskytovatel: Grantová agentura České republiky
Development of Fluorescent Probes for Methionine Sulfoxide Reductase and Beyond (GJ17-25897Y)
The aim of this project is to develop a fluorescent probe for methionine sulfoxide reductase. Methionine sulfoxide reductase plays a pivotal role in cellular redox signaling and its importance have been implicated in a number of dis...
CC - Organická chemie
- 2017 - 2019 •
- 4 452 tis. Kč •
- 4 452 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 2017 - 31. 12. 2019
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Grantová agentura České republiky
Bimetallic and trimetallic fluorescent nanoclusters synthesis, physico-chemical properties, and application in bio-imaging (GA19-03207S)
This project deals with metal nanoclusters possessing fluorescent properties. By a convenient combination of two and/or three metals, fluorescent as well as magnetic properties of particular components can be exploited; a (multi)bim...
Nano-materials (production and properties)
- 2019 - 2022 •
- 7 418 tis. Kč •
- 7 418 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 2019 - 30. 6. 2022
Uznané náklady
Podpora ze státního rozpočtu (100%)
Poskytovatel: Grantová agentura České republiky
Development of fluorescent probes for determination of content, localization and trafficking of cholesterol within eukaryotic cells (GA17-02836S)
to prepare a new generation of fluorescent probes based on specific features of the molecular recognition of cholesterol. New filipin derivatives and novel fluorescent alfa advanced microscopic techniques. Based on the obt...
CB - Analytická chemie, separace
- 2017 - 2021 •
- 6 684 tis. Kč •
- 6 015 tis. Kč •
- GA ČR
Řešení projektu: 1. 1. 2017 - 31. 12. 2021
Uznané náklady
Podpora ze státního rozpočtu (90%)
Poskytovatel: Grantová agentura České republiky
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