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36 903 (0,1s)

Výsledek výzkumu

High-yield expression of OspC protein from B. burgdorferi, isolation of monoclonal antibodies and epitope mapping

The aim of our study was to prepare recombinant forms of OspC protein (Borrelia burgdorferi s.l.) relevant for the Czech Republic and prepare a set of monoclonal antibodies with a high affinity to OspC protein and determine recognised epitopes

EB - Genetika a molekulární biologie

  • 2016
  • D
Výsledek výzkumu

Cancer vaccines: single-epitope anti-idiotype vaccine versus multiple-epitope antigen vaccine

In this study, we compared the immunogenicity and tumor-protective activity of anti-idiotypic antibodies mimicking a single tumor-associated epitope and tumor-associated antigen expressing multiple potentially immunogenic epitop...

FD - Onkologie a hematologie

  • 2000
  • Jx
Výsledek výzkumu

The expression of HNK-1 epitope and transcription factor Pax-3 in cranial neural crest cells of the chick

anatomy...

EA - Morfologické obory a cytologie

  • 2001
  • Jx
Výsledek výzkumu

Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv

benthamiana plants on the amount of transiently expressed fussion gene coding for the Human papillomavirus type 16 (HPV-16) epitopes fused to the sequence of recombinant Potato virus A coat protein (ACP). Epitope derived f...

GF - Choroby, škůdci, plevely a ochrana rostlin

  • 2008
  • Jx
Výsledek výzkumu

Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles usnig Potato Virus X-based vector

The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was express...

GF - Choroby, škůdci, plevely a ochrana rostlin

  • 2008
  • Jx
Výsledek výzkumu

New positions for peptide presentation in Potato virus X capsid protein

. The immunological characteristics of expressed epitopes were assessed by Western blot using both biotechnology, mostly for transient expression of desired peptides fused to the N-terminus bacterial expression of ...

EE - Mikrobiologie, virologie

  • 2015
  • Jx
  • Odkaz
Výsledek výzkumu

Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector

The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was express...

EB - Genetika a molekulární biologie

  • 2008
  • Jx
Výsledek výzkumu

Optimum storage conditions for produkt of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector

(ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprote...

GF - Choroby, škůdci, plevely a ochrana rostlin

  • 2008
  • Jx
Výsledek výzkumu

Expression plasmid vector for expression of human ATP5E fusion protein with V5 epitope and 6xHis tag for detection and purification

Plasmid vector for production of ATP5E subunit of human mitochondrial ATP-synthase with V5 epitope and 6xHis tag for detection and purification was constructed by recombinant molecular cloning methods. The methodology was optimized, that ena...

EB - Genetika a molekulární biologie

  • 2008
  • Gfunk
Výsledek výzkumu

Optimum storage conditions for product of transiently expressed epitopes of Human papillomavirus using Potato virus X -based vector

protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion withACP while an epitope derived from E7 on...

EE - Mikrobiologie, virologie

  • 2008
  • Jx
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