Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5? in Bioaerosols

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00000493%3AG41__%2F11%3A%230000388" target="_blank" >RIV/00000493:G41__/11:#0000388 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5? in Bioaerosols
Original language description
The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5? in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108?CFU/mL within 30?min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector.The level of 150?CFU/L in air was clearly indicated (20?min analysis including 5?min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/OVVTUO2008001" target="_blank" >OVVTUO2008001: Nanotechnology for immunosensor-based detection of bio-aerosols</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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